The role of bronchial epithelial-mesenchymal transformation and the effect of glucocortiod on it in air-way remodling of asthma
10.3760/cma.j.issn.1673-4912.2015.10.007
- VernacularTitle:支气管上皮间质转化在哮喘气道重塑中的作用及糖皮质激素对其影响
- Author:
Qi CHENG
;
Yunxiao SHANG
;
Miao LI
;
Qianlan ZHOU
;
Haihui SUN
- Publication Type:Journal Article
- Keywords:
Asthma;
Airway remodling;
Bronchial epithelial-mesenchymal transformation;
Glucocorticoid
- From:
Chinese Pediatric Emergency Medicine
2015;22(10):693-698
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate wether bronchial epithelial-mesenchymal transformation (EMT)take part in airway remodling in asthmatic mice,and the effect of inhaled glucocorticoid to it. Methods Thirty BALB /c mice were randomly divided into control group(n =10),asthma group(n =10) and budesonide group(n =10).The qualitity of serum OVA-sIgE was measured to vertify asthma modle. Bronchial airway thickness collagen deposition area was analyzed by HE and Masson staining to test the level of airway remodling.ELISA was used to test the quantity of transforming growth factor(TGF)-βin bron-choalveolar lavage fluid(BALF).Immunohistochemical assay was used to observe fibroblast specific protein-1 (FSP-1 )expressing area.Western blot and realtime PCR were performed to analyze the protein and mRNA expression of E-caderin,Vimentin and FSP-1 .Results The expression of OVA-sIgE,bronchial airway thick-ness,the collagen deposition area were significantly higher in asthma group than those in control group,while the above index in BUD group were allivated compared to those in asthma group(P <0.01 ,respectively). The expression of TGF-βin BALF increased and the expression of FSP-1 located in bronchial epithlium,the quantity of E-caderin significantly decreased,and the quantity of Vimentin and FSP-1 increased by Western blot and realtime PCR in asthma group,compared to those in control group(P <0.01 ).While the level of E-caderin increased(P <0.01 ),TGF-βand Vimentin reduced partly in BUD group(P <0.05 ),and there was no differece in the level of FSP-1 between BUD group and asthma group(P >0.05).Conclusion Lung tissue EMT take part in airway remodling of asthma,which is mainly focus on bronchial epithlium.The effect of traditional inhaled budesonide can not heal the bronchial EMT in asthma.
