Qualitation and quantitation of some ingredients in Qianliean Capsules

VernacularTitle:前列安胶囊部分中药成分定性定量研究
Author: Anfu TANG ; Yunyan XU ; Lei GONG ; Enzhong CUI ; Hao TANG ; Weidong LI
Publication Type:Journal Article
Keywords: Qianliean Capsules; Thin-layer chromatography; High-performance liquid chromatography; Radix Salviae milti-orrhizae; Rhizoma curcumae; Glycyrrhiza uralensis
From: Journal of Medical Postgraduates 2015;(11):1185-1188
CountryChina
Language:Chinese
Abstract: Objective The existing Quality Standards for Qianliean Capsules lack the quantitation control item and therefore cannot completely reflect the quality of the preparation .This study aimed to establish the methods for qualitation and quantitation of Qianliean Capsules . Methods Thin-layer chromatography ( TLC) was used for the qualitative identification of Radix Salviae miltior-rhizae, Rhizoma curcumae, and Glycyrrhiza uralensis in Qianliean Capsules .The content of hesperidin was determined by high-per-formance liquid chromatography ( HPLC) , and the method of determination was systematically evaluated . Results TLC spots were clear with a strong specificity .The content of hesperidin showed a good linear relationship within the range of 11 .56-310 .00μg/mL (r=0.999 5), with a mean recovery rate of 99.55% and relative standard deviation of 1.93%. Conclusion The qualitation and quantitation methods we established were simple , accurate and reliable , with a good reproducibility , and can be used for the quality control of Qianliean Capsules .