The role of CD19+CD24hiCD27hi regulatory B cells in the severity assessment of acute pancreatitis
10.3760/cma.j.issn.0254-5101.2015.09.010
- VernacularTitle:CD19＋CD24 hi CD27 hi调节性 B 细胞预测急性胰腺炎严重程度的作用研究
- Author:
Liannyu QIU
;
Qinhua YU
;
Huan WANG
;
Sujie ZHENG
;
Yonglie ZHOU
;
Qian LI
- Publication Type:Journal Article
- Keywords:
Acute pancreatitis;
Regulatory B cell;
Flow cytometry;
Interleukin-10;
Costimulatory molecule
- From:
Chinese Journal of Microbiology and Immunology
2015;(9):684-689
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the prognostic value of regulatory B cells (Bregs) in patients with acute pancreatitis .Methods Flow cytometry analysis was performed to detected the percentages of CD19+IL-10+and CD19+CD24hiCD27hi Bregs in peripheral blood samples collected from patients with acute pancreatitis (36 cases with mild acute pancreatitis and 15 cases with severe acute pancreatitis ) as well as the surface costimulatory molecules including CD80 and CD86 on CD19+CD24hiCD27hi Bregs.Their correlations with lymphocytes and C-reactive protein ( CRP) were further analyzed .Results The numbers of lympho-cytes, CD19+lymphocytes, CD19+IL-10+and CD19+CD24hi CD27hi Bregs in peripheral blood samples col-lected from patients with severe and mild acute pancreatitis as well as the mean fluorescence intensities ( MFI) of CD80 and CD86 were significantly lower than those from healthy subjects .Compared with patients with mild acute pancreatitis , the numbers of lymphocytes and CD 19+lymphocytes , the absolute numbers of CD19+IL-10+and CD19+CD24hiCD27hi Bregs as well as the mean fluorescence intensities (MFI) of CD80 and CD86 in patients with severe acute pancreatitis were significantly decreased .The percentages of CD19+IL-10+and CD19+CD24hiCD27hi Bregs in patients with mild acute pancreatitis were significantly increased af-ter an initial drop , but in patients with severe acute pancreatitis those values were continuously decreased along with the disease progression .The percentage of CD19+IL-10+Bregs was positively correlated with the percentage of CD19+CD24hiCD27hi Bregs and the absolute number of CD19+lymphocytes, but was negatively correlated with CRP .Conclusion The abnormal number and function of CD 19+IL-10+and CD19+CD24 hi CD27hi Bregs might be one of the important reasons causing immune dysfunction in patients with acute pan -creatitis.
