Effects of octreotide on the apoptosis of human HSCs and expression of Bcl-2/Bax in HSCs
10.3969/j.issn.1006-5725.2015.20.017
- VernacularTitle:奥曲肽对人肝星状细胞凋亡及Bcl-2／Bax表达的影响
- Author:
Chunyang LI
;
Liping JIA
;
Lei SHI
;
Xian ZHOU
- Publication Type:Journal Article
- Keywords:
Octreotide;
Hepatic stellate cells;
Apoptosis;
Bcl-2;
Bax
- From:
The Journal of Practical Medicine
2015;(20):3340-3342
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of octreotide on the apoptosis of human hepatic stellate cells (HSCs) and expression of Bcl-2/Bax in HSCs,and to reveal the mechanism underlying octreotide against hepatic fibrosis. Methods HSCs lines (HSC-LX2) were incubated with different concentrations of octreotide for 24 and 48 hours. Cell apoptosis was evaluated by Fitc-tunel fluorescence staining. Bcl-2 and Bax protein exoression in HSC-LX2 was detected by immunocytochemistry. Meanwhile, Bcl-2 protein of HSC-LX2 were detected by Western blot assay. Results Octreotide could promote the apoptosis of HSC-LX2, and the apoptosis rate was significantly increased with the concentration of octreotide(P < 0.05). The HSC-LX2 were incubated with the same concentration of octreotide for 24 and 48 hours, the cell apoptosis rate of 48-hour octreotide treatment was significantly higher than that of 24-hour octreotide treatment (P < 0.05). The immunocytochemistry result indicated that octreotide could significantly decrease Bcl-2 expression and increase Bax expression in HSC-LX2 (P<0.05); Western blot assay showed that octreotide could also significantly inhibit Bcl-2 expression in HSC-LX2 (P<0.05). Conclusions Octreotide could induce the apoptosis of HSCs in a dose-and time-dependent manner, the mechanism of octreotide inducing HSCs apoptosis might be associated with down-regulation of Bcl-2 and upregulation of Bax in HSC.
