Effects of Diosgenin on VEGF and AP-1 Expression in Synovial Tissues of CIA Rats
10.11842/wst.2015.09.009
- VernacularTitle:中药薯蓣皂苷元对CIA大鼠滑膜组织VEGF 及AP-I表达的影响＊
- Author:
Yachun GUO
;
Xiujun LIANG
;
Yaxian GAO
;
Hongru SONG
- Publication Type:Journal Article
- Keywords:
Vascular endothelial growth factor;
PCR;
diosgenin;
c-fos;
c-jun
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2015;(9):1801-1805
- CountryChina
- Language:Chinese
-
Abstract:
This paper was aimed to study effects of diosgenin on expressions of activator protein-1 (AP-1) and vascular endothelial growth factor (VEGF) in synovial tissues of collagen-induced arthritis (CIA) rats induced by bovine type II collagen, in order to investigate the possible mechanism of herbal medicine diosgenin in the treatment of rheumatoid arthritis (RA). After the CIA rats models were successfully established, rats were randomly divided into the blank control group, CIA model group, diosgenin group, and positive medicine control (tripterygium) group. The in situ hybridization was used to detect the expressions of AP-1 (c-fos and c-jun) in synovial tissues of the knee joint. The real-time PCR was used to detect the VEGF mRNA expression in synovial tissues of rats’ knee joints. The results showed that c-jun and c-fos, VEGF mRNA expressions in synovial tissues of rats’ knee joints were obviously higher than that of the blank control group (P<0.01). After treatment of diosgenin and tripterygium, the expressions of c-jun and c-fos, VEGF mRNA were significantly reduced (P<0.01). It was concluded that diosgenin may regulate the expression of VEGF in synovial tissues through c-jun and c-fos of AP-1 in order to inhibit synovial angiogenesis for the treatment of RA.
