Genetic Identification of 32 Different Origins and Categories of Commodity Edible Bird’s Nest

VernacularTitle:32种不同产地与品种商品燕窝的 DNA基源鉴定＊
Author: Fengyun WANG ; Liang HAN ; Xiaoping LAI
Publication Type:Journal Article
Keywords: Edible bird’s nest; DNA barcoding; Cytb; genetic identification
From: World Science and Technology-Modernization of Traditional Chinese Medicine 2015;(9):1876-1882
CountryChina
Language:Chinese
Abstract: This study was aimed to quickly and accurately identify different origins and categories of commodity edible bird’s nest (EBN) using DNA barcoding technique, in order to reveal its genetic differences. The total genomic DNA was isolated from the EBN samples. And Cytb gene sequences were amplified and sequenced by PCR. Then, 32 sequences were aligned and analyzed with DNAStar and MEGA 6.0 software. NJ phylogenetic tree was constructed. The nearest distance was calculated. The results showed that the original species of 32 samples of EBN were identified.Aerodramus fuciphagus was the genetic origin of 23 white nest samples. AndAerodramus fuciphagus germaniwas the genetic origin of the other 8 samples. The origin of black nest sample wasAerodramus maximusorAerodramus maximus lowi. It was concluded that the genetic origin of different EBN categories was variant. The identification of EBN’s origin species with Cytb sequence was quick and accurate.