Rapid Determination of Total Flavonoids in Chrysanthemum of Different Processing Methods by Near Infrared Spectroscopy
10.11842/wst.2015.09.035
- VernacularTitle:基于NIRS的不同产地加工方法野菊花药材中总黄酮的快速测定＊
- Author:
Xiaoli HU
;
Yan BAI
;
Jingwei LEI
;
Diwen ZHANG
;
Min HAO
- Publication Type:Journal Article
- Keywords:
near infrared spectroscopy;
different processing methods;
Chrysanthemum;
total flavonoids;
rapid determination
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2015;(9):1943-1948
- CountryChina
- Language:Chinese
-
Abstract:
This paper was aimed to study the method of rapid determination of total flavonoids in Chrysanthemum of different processing methods by near infrared spectroscopy (NIRS). The Chrysanthemum was dried by three different processes. The three methods were directly drying, drying after steamed and drying after fry, respectively. The determination of total flavonoids in Chrysanthemum by different processing methods was produced by using UV-Vis spectrophotometry. Collecting the NIRS spectra of Chrysanthemum, the quantitative analysis model of total flavonoids content in Chrysanthemum of different processing methods was established by partial least square (PLS) and the model was validated. The correlation coefficient (R2), the root-mean-square error of calibration (RMSEC) and the root-mean-square error of prediction (RMSEP) were 0.996 19, 0.104 and 0.168, respectively. The correlation coefficient of predication (r) was 0.979 3 which state that the prediction was accurate. The method of NIRS had the advantage of fast determination, simple operation and high accuracy of prediction, and could be used for rapid determination of total flavonoids content in Chrysanthemum of different processing methods.
