ox-LDL inhibits endothelial differentiation of bone marrow mesenchymal stem cells of rats
- VernacularTitle:ox-LDL抑制大鼠MSCs向内皮分化
- Author:
Huichao SUN
;
Lingjuan LIU
;
Lei ZHANG
;
Min ZHENG
;
Jing ZHU
;
Zhenguo LIU
;
Jie TIAN
- Publication Type:Journal Article
- Keywords:
oxidized low density lipoprotein;
bone marrow mesenchymal stem cells;
endothlial cells;
differentiation
- From:
Basic & Clinical Medicine
2015;(1):26-32
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate that ox-LDL inhibits endothelial differentiation of bone marrow mesenchymal stem cells (MSCs) in rats and its underlying mechanism .Methods Cultured MSCs were divided into four groups:blank groups , ox-LDL groups ( 5 μg/mL ox-LDL ) , ox-LDL +NAC groups ( 5 μg/mL ox-LDL and pre-treated NAC), and negative LDL groups (5 μg/mL nLDL).Cell morphology, endothelial marker and differentiation effi-ciency as well as signal of oxidative and pathway protein were detected after induction by Western blot , real-time PCR.Results MSCs can differentiate into endothelial cells with the expression of endothelial marker vWF , Flk-1 and CD31 at the mRNA and protein level , vascular morphology , ox-LDL obvious inhibited endothelial differentia-tion of MSCs ( P<0.05 ) , but NAC can reverse the inhibition , the amount of ROS in ox-LDL groups was higher than that in ox-LDL+NAC groups ( P <0.05 ) , The expression of phosphorylated Akt decreased distinctly after treatment with ox-LDL( P<0.05 ) , NAC can stimulated its expression close to normal .Conclusions ox-LDL can inhibit endothelial differentiation of MSCs via ROS , NAC in this procese shows inhibition to effect of ox-LDL and Akt signaling also played a critical role .
