Different expression of miRNA-449 a in lung cancer and precancerous tissue
- VernacularTitle:miRNA-449 a在人肺癌及癌旁组织中的差异表达
- Author:
Jianyong LIN
;
Yibin DENG
;
Yanhong LUO
;
Xiaochan LU
;
Yongzhi HUANG
- Publication Type:Journal Article
- Keywords:
lung neoplasms;
miRNA-449a;
tumor biomarker;
polymerase chain reaction;
fluorogenic quantitative
- From:
Basic & Clinical Medicine
2015;(1):44-47
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and biological function of miRNA-449 a in lung cancer . Methods A case-control study was conducted in 58 patients diagnosed with lung cancer ( carcinoma and adeno-carcinoma) and normal tissue closely adjacent to tumor.MiRNA-449a simulation was designed and synthesized, was dissolved into two different concentrations as 10 and 20 mg/mL.The expression of miRNA-449a in lung cancer tissues and matched normal tissues were detected by Real time PCR .The expression of luciferase gene was detected by chemiluminescence technique.MiRNA-449a mimics on cell apoptosis was evaluated by MTT assay . Results The mean tissues expression levels of miRNA-449 a in squamous carcinoma group and adenocarcinoma group were 1.48 ±1.63 and 1.52 ±1.54 respectively, and were significantly lower than in control group (2.74 ± 1.55 ) ( P<0.01 ) .The average intensity of fluorescent protein in 10 mg/mL group and 20 mg/mL group were 2 115 ±168 and 1 352 ±159 respectively , and were significantly lower than that in control group ( 4 975 ±115 ) ( P<0.01 ) .Conclusions MiRNA-449 a was down-regulated expression in lung cancer and induced apoptosis .
