Up-regulation of miR-200 a attenuates TGF-β1-induced activation and collagen synthesis in rat pancreatic stellate cells
- VernacularTitle:上调miR-200 a的表达减弱TGF-β1刺激的大鼠胰腺星状细胞活化和胶原合成
- Author:
Youli ZHANG
;
Guoying WANG
;
Yi ZHAO
;
Ping LI
;
Xin LIU
;
Xin NI
;
Min XU
- Publication Type:Journal Article
- Keywords:
TGF-β1;
PSCs;
miR-200 a;
fibrosis
- From:
Basic & Clinical Medicine
2015;(1):48-53
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of miR-200 a mimic on transforming growth factor β1-mediated acti-vation and collagen secretion of rat pancreatic stellate cells .Methods PSCs were isolated and cultured from pan-creatic tissue and identified by desmin , GFAP and α-SMA immunofluorescence staining .PSCs of 2nd generation were divided into control group , TGF-β1 group, TGF-β1+miR-NC group and TGF-β1+miR-200a mimic group.α-SMA and collagen Ⅰ protein were measured by Western blot and immunofluorescence staining .The mRNA ofα-SMA and collagen Ⅰ and the expression of miR-200a were detected by quantitative real-time PCR.Results TGF-β1 stimulates the activation of PSCs and promote collagen synthesis in time-dependment manner ( P<0.05 ) . After transfection of the mimic , treating with the same concentration of TGF-β1, the expressions of protein and mR-NA of both α-SMA and collagen Ⅰ decreases significantly ( P<0.01 ) .Conclusions Over-expression of miR-200 a significantly attenuates α-SMA activity and further affects the collagen synthesis of TGF-β1-dependent activa-tion of PSCs.The mechanisms are potentially related to the biological effects of TGF-β1.
