Detection of the levels of long non-coding RNAs including NEAT1 and MALAT1 in peripheral blood samples from patients with HIV-1 infection and their relationships with disease progression
10.3760/cma.j.issn.0254-5101.2015.09.014
- VernacularTitle:HIV-1感染者外周血长链非编码 RNA NEAT1和 MALAT1水平检测及其与疾病进展的关系
- Author:
Hongfeng YAO
;
Yujia GUO
;
Fangjun LUO
- Publication Type:Journal Article
- Keywords:
Long non-coding RNA;
Nuclear-enriched autosomal transcript 1 ( NEAT1);
Metasta-sis associated lung adenocarcinoma transcript 1 ( MALAT1 );
HIV-1;
Highly active antiretroviral therapy
- From:Chinese Journal of Microbiology and Immunology
2015;(9):698-702
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of two long non-coding RNAs ( lncRNAs ) during HIV-1 infection, which were nuclear-enriched autosomal transcript 1 (NEAT1) and metastasis asso-ciated lung adenocarcinoma transcript 1 ( MALAT1 ) , and their relationships with disease progression . Methods Fifty-nine patients with HIV-1 infection and 21 healthy subjects were recruited in this study , of which 31 patients were highly active antiretroviral therapy ( HAART)-na?ve and 28 patients received HAART for more than one year with undetectable viral loads .Total RNAs were extracted from PBMC and plasma samples, respectively.The levels of NEAT1 and MALAT1 were detected by quantitative real time polymer-ase chain reaction .Results The levels of NAET1 and MALAT1 in PBMC from HAART na?ve patients were 3 to 5 times higher than those in healthy subjects (P<0.01).The levels of NAET1 and MALAT1 in PBMC from HAART treated patients were similar to those in healthy subjects .The levels of NEAT1 in plasma sam-ples from patients with HIV-1 infection were lower than those in healthy subjects , and were positively corre-lated with CD4+T cell counts (P<0.01).No significance differences in the levels of MALAT1 in plasma samples were found between those with and without HIV-1 infection (P>0.05).Conclusion This study suggested that NEAT 1 and MALAT1 might be involved in the disease progression in patients with HIV-1 in-fection.The level of NEAT1 in plasma could be used as a potential biomarker of HIV-1 infection.
