HPLC Determination of Five Flavonoids Ingredients in Epimedium Flavonoids Capsule
10.11842/wst.2015.09.011
- VernacularTitle:HPLC法同时测定淫羊藿总黄酮胶囊中5种黄酮类成分＊
- Author:
Ruihua LI
;
Xiaoqian XU
;
Xiaoqiang ZHANG
;
Ying LI
;
Yun WU
;
Gang DING
;
Zhenzhong WANG
;
Wei XIAO
- Publication Type:Journal Article
- Keywords:
HPLC;
epimedinA;
epimedinB;
epimedinC;
epimedium glycoside;
baohuosideI
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2015;(9):1813-1817
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to establish an HPLC method for the content determination ofepimedin A, epimedinB,epimedinC,epimedium glycoside,baohuosideI in epimedium flavonoids capsule. The elusion was performed on an Eclipse Plus C18column (250 mm× 4.6 mm, 5μm). The mobile phase was composed of acetonitrile and water with a gradient elution. The flow rate was set at 1 mL·min-1. The detection wave length was set at 270 nm. The column temperature was 25℃. The results showed that the linear ranges of epimedin A,epimedinB,epimedinC,epimedium glycoside,baohuosideI were 3.10-62.00μg·mL-1, 5.70-114.00μg·mL-1, 9.14-182.80μg·mL-1, 15.20-304.00μg·mL-1, and 1.56-31.20μg·mL-1, respectively. The correlation coefficientr was more than 0.999 3. The average recoveries were 101.06% (RSD = 1.05%,n = 6), 100.78% (RSD = 1.08%,n = 6), 99.17% (RSD = 1.14%,n = 6), 100.23% (RSD = 0.68%,n = 6), and 99.09% (RSD = 1.30%,n = 6), respectively. This experiment was precise, reproducible and stable. It was concluded that the method was simple and accurate, which provided a certain reference value for the multi-component assaying of epimedium flavonoids capsule.
