Determination of Isogarcinol in Seven Guttiferae Plant Species by HPLC

VernacularTitle:HPLC测定7种藤黄科植物中异藤黄酚的含量＊
Author: Zijin LIU ; Yaoying JIANG ; Yuxia ZHANG ; Yingying LI ; Jing ZHOU ; Donghai LI ; Xiaobo YANG ; Juren CEN
Publication Type:Journal Article
Keywords: HPLC; guttiferae; Isogarcinol; determination
From: World Science and Technology-Modernization of Traditional Chinese Medicine 2015;(9):1872-1875
CountryChina
Language:Chinese
Abstract: This study was aimed to establish a HPLC method for the determination of isogarcinol. Daojin Inertsil WP300 C18 (4.6 mm× 150 mm, 5μm) was employed with methanol and water (75?25) as the mobile phase. The flow rate was 1.0 mL·min-1. The column temperature was set at 40°C. The detection wavelengthγ was set at 277 nm. The results showed that the linear range of isogarcinol was 0.005 7-0.039 9μg. The average recovery rate was 99.58%. The relative standard deviation (RSD) was 1.25%. The contents of isogarcinol inGarcinia mangostana,Garcinia oblongifolia,Garcinia oligantha,Cratoxylum cochinchinense andCalophyllum membranaceum were 0.285%, 0.199%, 0.857%, 0.161% and 0.006%, respectively. Isogarcinol was not detected inCratoxylum formosum orCalophyllum inophyllum. It was concluded that the method was convenient, accurate with high sensitivity, good stability and repeatability. It can be used for determination of isogarcinol content in Chinese herbal medicine.