The role of the hydrogen sulfide signal system in rat bone marrow-derived mesenchymal cells osteogenesis under tensile stress
10.3969/j.issn.1006-5725.2015.20.002
- VernacularTitle:张应力下大鼠骨髓间充质细胞成骨分化过程中硫化氢信号系统的作用
- Author:
Xiaowen JIANG
;
Yi ZHAN
;
Xiaosheng FAN
;
Xuan DENG
- Publication Type:Journal Article
- Keywords:
Tensile stress;
Bone marrow-derived mesenchymal cells;
Hydrogen sulfide
- From:
The Journal of Practical Medicine
2015;(20):3291-3294
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the impact of hydrogen sulfide signal system during the process of rat bone marrow-derived mesenchymal cells osteogenesis under tensile stress. Methods After the H2S signal system of cell rooms regulated by tool drugs (Group A with propargylglycine;Goup B with PBS;Group C with GYY3147), 4 000 μ strain tensile stress were applied on rat BMMCs by four-point bending apparatus for 60 minutes. Four hours later, the H2S signal system and cystathionine-γ-lyase were detected. Meanwhile, the change of the alkaline phosphate, osteocalcin, procollagen typeⅠN-terminal propeptide, and runt-related transcription factor 2 mRNA level were also examined to evaluate the osteogenic ability. Results With the increase of H2S expression, the osteogenic capacity gradually increased, while the osteogenic capacity was compromised after the endogenous cystathionine-γ-lyase was inhibited. Conclusion The H2S signal system plays an important role during BMMCs osteogensis under tensile stress. The up-regulation expression of H2S may promote osteogenesis during distraction osteogenesis.
