The effect of curcumin on inflammation on Propionibactierium acnes-induced THP-1 cell
10.3969/j.issn.1006-5725.2015.20.003
- VernacularTitle:姜黄素对痤疮丙酸杆菌诱导的炎症反应的作用
- Author:
Qiaoli GU
;
Yan CAI
;
Huilin YANG
;
Qin SHI
- Publication Type:Journal Article
- Keywords:
Curcumin;
Propionibactierium acnes;
TLR2
- From:
The Journal of Practical Medicine
2015;(20):3295-3297
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the anti-inflammatory mechanism by which curcumin affects TNF-αand IL-8 production in Propionibactierium acnes-induced THP-1 cells. Methods THP-1 cell viability was determined by CCK8 assay. The productions of TNF-α and IL-8 were detected by ELISA assay. Total RNA and proteins were collected for real-time PCR and Western blot analysis, respectively. Results Curcumin didn′t significantly affect the cell viability at 12 h. It decreased Propionibactierium acnes-induced productions of TNF-αand IL-8 in THP-1 cells. Moreover, it decreased TLR2, NF-κB p65, and P-NF-κB p65 expressions in THP-1 cells. Conclusions Curcumin may reduce TNF-α and IL-8 expressions in Propionibactierium acnes-induced THP-1 cells by inhibiting TLR2/NF-κB signaling pathway.
