miR-30 a suppresses migration, invasion and vitality of human osteosarcoma cell line 143 B
- VernacularTitle:miR-30 a抑制人骨肉瘤细胞143 B的迁移、侵袭和活力
- Author:
Ruyi ZHANG
;
Fang HE
;
Jing WANG
;
Fang DENG
;
Qiying LI
;
Qiong SHI
- Publication Type:Journal Article
- Keywords:
miR-30a;
human osteosarcoma cell line;
RUNX2
- From:
Basic & Clinical Medicine
2015;(1):12-16
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of miR-30a on human osteosarcoma cell 143B in migration,invasion andcellviability.Methods 143BcellswereinfectedortransfectedwithrecombinantadenovirusmiR-30a(Ad-miR30a) and miR-30a inhibitor respectively .Wound healing assay was performed to detect the cell healing ability ( P<0.05 ) .Cell migration and invasion ability were determined by Transwell assay ( P<0.05 ) .The cell viability was analyzed by MTT assay ( P<0.01 ) .Real-time quantitative PCR was performed to analyze the expression of RUNX2 mRNA level and confirmed the adenovirus miR-30a expressed in 143B cells.The expression of RUNX2 was analyzed by Western blot .miR-30a target to RUNX2 was verified by luciferase reported gene assay .Results The ability of migration and invasion was suppressed in osteosarcoma cell 143B by overexpression miR-30a,and the cell viability also decreased .After the endogenous miR-30 a being inhibited , the cell motility and invasion enhanced and the cell viability was promoted .The RUNX2 protein decreased after overexpression miR-30 a as compared with controlgroup.TheluciferaseactivityofRUNX2decreasedbyaddingmiR-30a.Conclusions 143Bcellmigration, invasion and viability were suppressed by miR-30a,and this process is potentially achieved via suppressing RUNX 2 protein expression .
