Synthesis and Characterization of a Novel ELF-97-based Fluorescent Probe for Hydrogen Peroxide Detection
10.11895/j.issn.0253-3820.150124
- VernacularTitle:基于ELF-97的新型H2 O2荧光探针的合成与性能研究
- Author:
Zijun CAI
;
Yongqing KUANG
;
Dan PAN
;
Wei LIU
;
Jianhui JIANG
- Publication Type:Journal Article
- Keywords:
Fluorescent probe;
2-( 2'-Hydroxy-5'-chlorophenyl )-6-chloro-4 ( 3 H )-quinazolinone;
Hydrogen peroxide
- From:
Chinese Journal of Analytical Chemistry
2015;(11):1671-1675
- CountryChina
- Language:Chinese
-
Abstract:
A novel 2-( 2’-hydroxy-5’-chlorophenyl )-6-chloro-4 ( 3 H )-quinazolinone ( ELF-97 )-based fluorescent probe (P1) for hydrogen peroxide was prepared from 5-chlorosalicylaldehyde, 4-(bromomethyl) phenylboronic acid and 2-amino-5-chlorobenzamide, and its structure was characterized by 1 H NMR, 13 C NMR and HRMS. Weak fluorescence intensity was observed at 425nm when the solution of probe P1 was excited by 360 nm UV light. After addition of H2 O2 , however, emission peak at 425 nm disappeared while strong peak emission at 515 nm appeared with the same excitation wavelength ( 360 nm ) . The fluorescence intensity at 515 nm was dependent on the concentration of H2 O2 in the linear response range of 5-45 μmol/L. The detection limit of H2O2 was 0. 1 μmol/L (S/N=3) and the recovery rates of added H2O2 in milk were in the range of 94 . 0%-106 . 0%. Probe P1 was potential to become a useful tool for rapid detection of hydrogen peroxide.
