Determination of 15+1 European Priority Polycyclic Aromatic Hydrocarbons in Edible Oil by Online Solid Phase Extraction and High Performance Liquid Chromatography
10.11895/j.issn.0253-3820.150300
- VernacularTitle:在线固相萃取-高效液相色谱法检测食用油中15+1种欧盟优控多环芳烃
- Author:
Chunlei WANG
;
Hualiang LIU
;
Yongjian MA
;
Liping RUAN
- Publication Type:Journal Article
- Keywords:
Online solid phase extraction;
Polycyclic aromatic hydrocarbons high performance liquid chromatography;
Edible oil
- From:
Chinese Journal of Analytical Chemistry
2015;(11):1743-1748
- CountryChina
- Language:Chinese
-
Abstract:
A novel method was developed for the determination of the 15+1 European priority polycyclic aromatic hydrocarbons in edible oil by online solid-phase extraction coupled with high performance liquid chromatography-ultraviolet/ fluorescence detection ( online-SPE-HPLC-UV/FL-D ) . The edible oil samples were diluted with isopropyl alcohol, and then filtered. The online extraction was performed on a solid phase extraction ChromSpher Pi column (80 mmí3 mm) and the separation was carried out on a C18 reversed-phase PAH column (250 mmí4. 6 mm i. d, 5μm) using ultraviolet detection at 220 nm and fluorescence detection. Isopropyl alcohol, acetonitrile and water were served as mobile phase in gradient elution. The results showed good linearity for the 15+1 polycyclic aromatic hydrocarbons with all the correlation coefficients (R2)>0. 99. The limits of detection ( LODs ) were between 0. 03 and 12. 23 μg/kg. The recoveries of the sixteen components in the three levels of spiked samples were in the range of 65 . 3%-110 . 5% with the relative standard deviation (RSD, n=6) from 0. 1% to 9. 8%.
