Analysis of Brain Pharmacokinetics of Hyperoside and 1,5-Dicaffeoylquinic Acid and Treatment Effects of Acanthopanax Senticosus Leaves on Cerebral Ischemia by On-line Microdialysis-Tandem Mass Spectrometry
10.11895/j.issn.0253-3820.150364
- VernacularTitle:刺五加叶药物成分脑内代谢动力学及治疗脑缺血作用的在线微透析-质谱分析
- Author:
Qianqian WANG
;
Yan ZHANG
;
Lulu MENG
;
Zifeng PI
;
Shu LIU
;
Fengrui SONG
;
Zhiqiang LIU
- Publication Type:Journal Article
- Keywords:
Acanthopanax senticosus leaves;
Neurotransmitters;
Cerebral ischemia;
Online microdialysis-tandem mass spectrometry
- From:
Chinese Journal of Analytical Chemistry
2015;(11):1754-1760
- CountryChina
- Language:Chinese
-
Abstract:
The brain pharmacokinetics of hyperoside and 1 , 5-dicaffeoylquinic acid and the effects of acanthopanax senticosus leaves on neurotransmitters in cerebral ischemic rat brain were investigated. In the study, acute incompleteness cerebral ischemia model was developed by ligating the bilateral common carotid arteries of rats. The differences of brain pharmacokinetics of hyperoside and 1,5-dicaffeoylquinic acid between control and cerebral ischemic rats were determined by online microdialysis coupled with MS/MS method. At the same time, the contents of glutamic acid (Glu), aspartic acid (Asp), γ-aminobutyric acid (GABA), serotonin (5-HT) , dopamine ( DA) , and acetylcholine( Ach) in rat hippocampus among different groups were determined simultaneously in MRM mode. ACE 5 C18-AR column was used for separation. Results showed that the online analytical method had excellent linearity (R2>0. 99). The accuracy and precision could meet the analysis requirement. Besides, the hyperoside and 1,5-dicaffeoylquinic acid penetrated the blood-brain barrier successfully and metabolized quickly, but the absorption reduced and elimination became faster in cerebral ischemic rats; the contents of Glu, Asp, GABA and DA in brain issue of cerebral ischemic rats decreased significantly ( p<0 . 01 ) and the level of Ach increased remarkably ( p<0 . 05 ) after a preventive medication with the extract of acanthopanax senticosus leaves for one week.
