Effect of YC-1 on the hypoxia-induced proliferation of HPASMCs and the P53 expression
- VernacularTitle:YC－1对低氧诱导人肺动脉平滑肌细胞增殖和P53表达的影响
- Author:
Mingxing LI
;
Deqi JIANG
;
Yan WANG
;
Yanjiao MA
;
Shanshan YU
;
Yong WANG
- Publication Type:Journal Article
- Keywords:
YC-1;
human pulmonary artery smooth muscle cells;
proliferation;
apoptosis;
P53
- From:
Basic & Clinical Medicine
2015;(10):1303-1307
- CountryChina
- Language:Chinese
-
Abstract:
Objective_To investigate the effects of hypoxia-inducible factor-1 alpha ( HIF-1α) inhibitor YC-1 on hy-poxia induced human pulmonary artery smooth muscle cells ( HPASMCs) proliferation, apoptosis and the expression of P53, and to explore the molecular mechanism in the processes.Methods_HPASMCs were cultured in DMEM me-dium supplemented with 10%FBS in vitro.Then divided them into four groups:normoxia, hypoxia and hypoxia+YC-1(0.01 and 0.05 mmol/L).Cell proliferation was measured by CCK-8 and apoptosis was detected by flow cytom-etry.The expressions of HIF-1αand P53 were tested by Western blot, and the mRNA expression of P53 was tested by RT-PCR.Results_Hypoxia can promote the proliferation of HPASMCs.Treatment of HPASMCs with different concentrations of YC-1 intervention for 24h obviously dropped proliferation rate (P<0.05), and the apoptosis rate increased significantly (P<0.05).YC-1 can also down-regulate the expression of HIF-1αand up-regulate the ex-pression of P53 significantly ( P<0.05 ) .Conclusions_YC-1 can inhibit hypoxia-induced HPASMCs proliferation and promote apoptosis, the mechanism is potentially related to the up-regulation of P53 expression.
