Recombinant AAV-IFNαinhibits HBV replication in vitro
- VernacularTitle:重组腺相关病毒表达干扰素α体外抑制乙肝病毒的复制
- Author:
Jiang ZHU
;
Lei GAO
- Publication Type:Journal Article
- Keywords:
hepatitis B virus;
adeno-associated virus;
interfron
- From:
Basic & Clinical Medicine
2015;(10):1331-1335
- CountryChina
- Language:Chinese
-
Abstract:
Objective_To identify the anti-HBV efficiency of AAV-IFNαby infecting HepG2.2.15 cell line, and to develop a new strategy of curing hepatitis B.Methods_Firstly,recombinant vector was packaged into AAV by three plasmids co-transfection method;then AAV-IFNαinfected HepG2.2.15 cell line, and samples were collected on the 1st, 3rd,6th and 9th day;thirdly, The time-course of HBV DNA,HBsAg and HBeAg were detected by qPCR and ELISA.The proliferation of HepG2.2.15 cells detected by MTT.Results_Recombinant AAV-IFNαcan significantly inhibit HBV replication and expression in vitro because of the proliferation of HepG2.2.15 cells inhibited by AAV6-IFNα( P<0.05) .Conclusions_AAV-IFNαis a potential antiviral product for hepatitis B.
