Dickkopf-1 inhibits vasculogenic mimicry formation of colon can-cer and relevant mechanism
10.3969/j.issn.1000-8179
- VernacularTitle:Dickkopf-1对结肠癌组织血管生成拟态形成的抑制作用及相关机制研究
- Author:
Lisha QI
;
Zhiyong LIU
;
Wangzhao SONG
;
Runfen CHENG
;
Baocun SUN
- Publication Type:Journal Article
- Keywords:
Dickkopf-1;
vasculogenic mimicry;
colon cancer;
VE-cadherin;
three dimensional culture
- From:
Chinese Journal of Clinical Oncology
2015;(20):1007-1011
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the inhibitory effect of Dickkopf-1 (Dkk1) on vasculogenic mimicry (VM) formation and the relevant mechanism. Methods:CD34-PAS dual staining and immunohistochemical staining were used to detect and analyze the re-lationship between VM existence and Dkk1 expression in 217 human colon cancer tissue samples;three dimensional (3D) culture was used to detect the influence of Dkk1 on tube structure formation and on VE-cadherin expression;a subcutaneous mouse xenograft mod-el was made to further validate the inhibitory role of Dkk1 on VM formation in vivo. Results:VM-positive samples indicated a lower expression of Dkk1(P<0.05);colon cancer cells with Dkk1 overexpression exhibited a decreased ability to form tube-like structure and a decreased expression of VE-cadherin;Dkk1 inhibited the VM-formation abilities of human colorectal carcinoma cell line xenograft tu-mor tissue. Conclusion:Dkk1 inhibits the VM formation of colon cancer.
