Chiral Separation and Identification of D,L-Histidine Based on Nanochannels Membrane Coupling with Surface Enhanced Raman Scattering Spectroscopy
10.11895/j.issn.0253-3820.150595
- VernacularTitle:基于纳米通道表面增强拉曼散射光谱分离检测组氨酸对映体的研究
- Author:
Tongsheng ZHONG
;
Zhifang YIN
;
Yue LIU
;
Shansheng HUANG
- Publication Type:Journal Article
- Keywords:
Gold nanochannel;
Chiral separation;
Surface enhanced Raman scattering spectroscopy;
Histidine
- From:
Chinese Journal of Analytical Chemistry
2015;(11):1695-1700
- CountryChina
- Language:Chinese
-
Abstract:
Gold nanochannels were prepared using Al2 O3 nanotubules membrane as the carrier and modified with chitosan by a classical N-(3-dimethylaminopropyl)-N-ethyl carbodiimide ( EDC)/N-hydroxysuccinimide ( NHS ) coupling reaction. The nanochannels were characterized by field emission scanning electron microscopy ( FESEM) , cyclic voltammetry and AC impedance method. The Au nanochannels modified with chitosan showed a chiral environment and can be used to separate histidine enantiomer. The effects of pore size and solution pH on the separation efficiency of histidine were investigated. To increase the detection sensitivity of D-, L-histidine, Ag nanoparticles were used to enhance the surface enhanced Raman scattering ( SERS) activity. The results showed that the chitosan-modified gold nanochannels can be used to separate chiral histidine based on this unique selective nanochannel membrane. L-Histidine and D-histidine were respectively detected by SERS at wavelengths of 1000 and 1590 cm-1 . The results showed that L-histidine and D-histidine were separated well in the mixture containing 200 μL of histidine, 100 μL of colloidal Ag and 100 μL of 80 mmol/L NaCl ( pH=7 . 59 ) with a separation efficiency of 4 . 91 .
