Determination of Naringenin and Apigenin in Premna fulva Craib.by HPLC
10.3870/j.issn.1004-0781.2015.11.018
- VernacularTitle:壮药战骨中柚皮素与芹菜素含测定量?
- Author:
Yong YE
;
Qiujie HUANG
;
Huagang LIU
;
Guangqiang CHEN
;
Yuhong NONG
;
Liting YANG
;
Jinhua ZHONG
- Publication Type:Journal Article
- Keywords:
Premna fulva Craib.;
Naringenin;
Apigenin;
Chromatography,high performance liquid;
Content determination
- From:
Herald of Medicine
2015;(11):1483-1485
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for determination of naringenin and apigenin in Premna fulva. Methods The SHISEIDO￣SPOLAR C18(250 mm×4.6 mm,5μm) was used as analytical column.The mobile phase consisted of methanol￣0.2% phosphoric acid (42:58) with isocratic elution at a flow rate of 1.0 mL.min-1 .The detection wavelength of naringenin and apigenin was 288 nm and 340 nm, respectively.Column temperature was set at 35 ℃ , the injection Volume was 10 μL. Results Naringenin and apigenin had a good linear relationship in the concentration range of 0.180 ~ 3.60 μg (r =0.999 9) and 0. 0052 ~ 0. 1040 μg ( r = 0. 999 9), respectively. Conclusion The method is accurate and reliable. It is appropriate for the quantitative determination of naringenin and apigenin in Premna fulva and its preparations.
