Effects of Ulinastatin on Renal Expression of Endothelin￣1 and Nitric Oxide in Ratswith Toxic Acute Kidney Injury
10.3870/j.issn.1004-0781.2015.11.004
- VernacularTitle:乌司他丁对急性肾损伤大鼠肾脏内皮素-1与一氧化氮的影响?
- Author:
Jianming SHEN
;
Yanyan DENG
;
Tingting YE
;
Rong GOU
;
Shaojiang TIAN
;
Liping WANG
;
Junfeng LI
- Publication Type:Journal Article
- Keywords:
Ulinastatin;
Injury,kidney,acute;
Endothelin-1;
Nitric oxide;
Nitric oxide synthase,induced
- From:
Herald of Medicine
2015;(11):1429-1433
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of ulinastatin on renal expression of endothelin￣1 and nitric oxide (NO) in rats with toxic acute kidney injury(AKI). Methods Twenty￣four male SD rats were randomly divided into the normal control group,model control group and treatment groups, with 8 rats in each group. Except normal control group, rats were subcutaneously injected with gentamicin (300 mg.kg-1 .d-1 ) for 3 days to establish a model of toxic AKI.Rats in the treatment group were intraperitoneally injected with a 7￣day course of ulinastatin (30 000 U.kg-1 .d-1 ) from the fourth day.While the other two groups were injected with 0.9% sodium chloride injection (3 mL.kg-1 .d-1 ). The serum level of creatinine and cystatin￣C,urinary concentration of kidney injury molecule￣1(Kim￣1) and neutrophil gelatinase￣associated lipocalin (NGAL), level of endothelin￣1 and NO,expression of endothelin￣1 mRNA,endothelial nitric oxide synthase (eNOS),induced nitric oxide synthase (iNOS),eNOS mRNA and iNOS mRNA in homogenate of renal tissues in each group were detected on the eleventh day. Results Compared with the normal control group,serum level of creatinine and Cystatin￣C,urinary concentration of Kim￣1 and NGAL,level of endothelin￣1 and NO,expression of endothelin￣1 mRNA,iNOS and iNOS mRNA in homogenate of renal tissues were higher in model control group (P<0.01, respectively), while which were lower in treatment group than those in model control group ( P < 0. 01, respectively). And no statistical significant difference of eNOS and eNOS mRNA expression in homogenate of renal tissues existed among the three groups. Conclusion Ulinastatin possesses curative role against rat with toxic AKI via down￣regulating renal expression of endothelin￣1,NO and iNOS.
