Protective Effects of Memantine on Isofluane￣induced Decrease of Proliferation in Neural Stem Cells
10.3870/j.issn.1004-0781.2015.11.005
- VernacularTitle:美金刚对异氟烷所致神经干细胞增殖能力减退的保护作用?
- Author:
Xin CHEN
;
Yilin ZHAO
;
Wei WANG
;
Jianfang ZHANG
;
Shiyong LI
;
Ailin LUO
- Publication Type:Journal Article
- Keywords:
Memantine;
Isoflurane;
Stem cells,neural;
Proliferation
- From:
Herald of Medicine
2015;(11):1433-1438
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effects of memantine on isofluane￣induced decrease of proliferation in neural stem cells ( NSCs) and the potential mechanisms in vitro. Methods Neural stem cells were isolated from rat hippocampi (postnatal day 1) and grew in culture. Cultured NSCs were randomly divided into the control ( Group Control), Vehicle (Group Vehicle), Isoflurane ( Group Iso), Isoflurane +Memantine ( Group Iso +Mem) and Isoflurane + Memantine +LY294002 groups (Group Iso+Mem+LY).Proliferation was assessed by cell counting and BrdU incorporation.Western blot was conducted to detect protein expression of phospho￣Akt. Results Compared with the control group,BrdU incorporation and phospho￣Akt expressions in neural stem cells significantly declined after 2.4% isoflurane exposure for 6 h (P<0.01).However, isofluane￣induced decrease of BrdU incorporation and phospho￣Akt expressions was attenuated by the treatments of memantine (P<0.01)). It was showed that Akt inhibitors LY294002 reversed the protective effects on neural stem cells proliferation by memantine(P<0.01). Conclusion The results suggest that memantine treatment might attenuate isofluane￣induced decrease of proliferation in neural stem cells via Akt signaling pathway.
