Effect of 2,3,4′,5-Tetrahydroxystilbene-2 -O -beta -D-glucoside on the Proliferation of Pulmonary Artery Smooth Muscle Cells In-duced by Hypoxia
10.11969/j.issn.1673-548X.2015.10.043
- VernacularTitle:二苯乙烯苷对低氧肺动脉平滑肌细胞增殖的影响
- Author:
Zhiyong ZHANG
;
Shuizhen YANG
- Publication Type:Journal Article
- Keywords:
Hypoxia;
Pulmonary artery smooth muscle cells;
2;
3;
4′;
5-Tetrahydroxystilbene -2-O-beta-D-glucoside;
Pro-liferation
- From:
Journal of Medical Research
2015;44(10):151-154
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of the 2,3,4′,5-Tetrahydroxystilbene -2-O-beta-D-glucoside on the prolif-eration of PASMCs induced by hypoxia , in orde to search new drugs for the treatment and prevention of hypoxic pulmonary vascular remod -eling.Methods 3%O2 hypoxia was used to induced the proliferation of PASMCs .After hypoxic and TSG treatment for 24h, cell growth was determined by cell counting kit -8 ( CCK-8 ) , cell cycle was analysed by flow cytometry , the mRNA expression of HIF -1αwas measured by quantitative real -time PCR, and the reactive oxygen species ( ROS) production was determined by the fluorescence micro-plate reader .Results TSG can block the proliferation of PASMCs through G 0/G1 to S phase of the cell cycle arrest without cell cytotoxic-ity.Further experiments showed that TSG blocking the proliferation of PASMCs was associated suppression the mRNA expression of HIF -1αand the production of intracellular ROS in hypoxia -stimulated-PASMCs.Conclusion TSG can inhibit the proliferation of pulmo-nary artery smooth muscle cells induced by hypoxia through suppression the mRNA expression of HIF -1αand the production of intracel-lular ROS.
