IL-13 inhibits differentiation of human nasal ciliated epithelial cells
10.16066/j.1672-7002.2015.10.011
- VernacularTitle:白细胞介素13抑制人鼻腔上皮纤毛细胞分化的研究
- Author:
Ming WANG
;
Jian JIAO
;
Luo ZHANG
- Publication Type:Journal Article
- Keywords:
Interleukin-13;
Epithelium;
Cilia;
Transcription Factors;
basal body biogenesis
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2015;(10):526-530
- CountryChina
- Language:Chinese
-
Abstract:
[ABSTRACT]OBJECTIVETo explore the role and mechanism of IL-13 in regulating the differentiation of ciliated cells on cultured human primary nasal epithelial cells.METHODSHuman nasal epithelial cells were cultured in vitro and treated by IL-13, the differentiation of epithelial cells was observed by western blot detection ofβ-tubulinⅣ and ELISA detection of MUC5AC; the percentage of ciliated and goblet cells was quantified by cytospin followed by immunohistochemical staining; the expression level of central ciliary genes was detected by real-time PCR.RESULTSCompared with control group, IL-13 treatment for 14 day significantly decreased the percentage of ciliated cells and expression ofβ-tubulinⅣ, additionally increased the goblet cells and the secretion of MUC5AC; IL-13 treatment significantly reduced the expression of ciliary key transcription factors such as FOXJ1, RFX2 and RFX3, as well as basal body biogenesis genes like CCNO and CETN2.CONCLUSIONIL-13 inhibits the differentiation of human nasal ciliated epithelial cells, which may be by down-regulating the expression of ciliary key transcription factors and basal body biogenesis proteins.
