Human insulin inhibits apoptosis and promotes proliferation of rat liver cell line BRL-3 A
- VernacularTitle:人胰岛素抑制大鼠肝细胞系BRL－3 A凋亡且促增殖
- Author:
Gaiping WANG
;
Shasha CHEN
;
Xiaofang LI
;
Jing YANG
;
Weiming ZHAO
;
Cuifang CHANG
;
Cunshuan XU
- Publication Type:Journal Article
- Keywords:
human insulin;
BRL-3A cells;
apoptosis;
cell cycle
- From:
Basic & Clinical Medicine
2015;(10):1320-1324
- CountryChina
- Language:Chinese
-
Abstract:
Objective_To study the effect of human insulin on cell cycle progression and apoptosis of rat liver cell line BRL-3A in vitro.Methods_MTT method was used to observe the effect of insulin on cell activity, and flow cytometry was used to detect cell apoptosis and cell cycle.qRT-PCR was used to evaluate the expression of related genes.Results_Human insulin induced the proliferation of BRL-3A cells in a dose-dependent manner ( P<0.05 or P<0.01);After 3 days treated by human insulin (500 nmol/L), the proportion of cells in G0/G1 phases re-markably decreased (P<0.05).Moreover, pro-apoptotic BAX was down-regulated (P<0.05), while cell prolif-eration-related gene CCNA2 was up-regulated (P<0.05).Conclusions_Human insulin may inhibit the apoptosis of BRL-3 A cell line and induce proliferation due to the down-regulated expression of BAX and up-regulated expres-sion of CCNA2 .
