The effect of Y-27632 on invasion and migration of gastric carcinoma cell line SGC-7901
- VernacularTitle:Y－27632抑制胃癌SGC－7901细胞系侵袭与转移
- Author:
Min ZHAO
;
Ying ZHOU
;
Jiangmei HUANG
;
Fang XIAO
;
Xiaochao LI
;
Hui ZHANG
;
Ruiji LIU
- Publication Type:Journal Article
- Keywords:
gastric carcinoma;
serum response factor(SRF);
Y-27632(ROCK inhibitor);
SGC-7901 cell line;
RNA interference
- From:
Basic & Clinical Medicine
2015;(10):1369-1374
- CountryChina
- Language:Chinese
-
Abstract:
Objective_To study the effect of Y-27632 on invasion and motility of SGC-7901 gastric carcinoma cells, and to find whether Y-27632 excerts the effect by attenuating SRF expression.Methods_SGC-7901 gastric carcinoma cells were divided into 3 groups:1)blank control group;2)Y-27632 group;3)siRNA-SRF-1107 group. Transfected siRNA-SRF or incubated by Y-27632 48 h.The effect of Y-27632 on proliferation suppressions of SGC-7901 gastric carcinoma cells was detected by CCK-8 assay.Cell invasion was examined by Transwell and wound healing test.The expression of SRF, ROCK1, E-cadherin, β-catenin, F-actin, MRTF-A and Cyclin D1 were detected by Western blot.Results_Y-27632 inhibited invasion (P<0.05)but had no effect on proliferation of SGC-7901 gastric carcinoma cells.Y-27632 reduced ROCK1, MRTF-A, F-actin, SRF protein expressions by 37.0%, 44.3%, 62.7%and 62.7%respectively, and E-cadherin protein expression was up-regulated by 2.64 folds(P<0.05).Conclusions_The inhibition of ROCK and up-regulation of E-cadherin by Y-27632 can inhibit the invasion and migration of SGC-7901 gastric carcinoma cells that is explained at least, in part, by attenuating SRF expression.
