Effects of Ginsenosides Rg3 on Vasculogenic Mimicry of Pancreatic Cancer Xenograft
10.11969/j.issn.1673-548X.2015.10.035
- VernacularTitle:人参皂甙 Rg3 对胰腺癌裸鼠皮下移植瘤血管生成拟态的研究
- Author:
Dandan JIANG
;
Jingqiang GUO
;
Liang CHEN
- Publication Type:Journal Article
- Keywords:
Pancreatic cancer;
Xenograft model of pancreatic cancer;
Vasculogenic mimicry;
Matrix metalloproteinase
- From:
Journal of Medical Research
2015;44(10):124-128
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of ginsenosides Rg 3 on vasculogenic mimicry of pancreatic cancer xenograft through the establishment of pancreatic cancer cell line SW -1990 subcutaneous xenograft model .Methods After pancreatic cancer xenograft in nude mice model beening established , All the mice were randomly divided into 4 groups and treated intraperitoneally ( IP) with saline and various concentrations (5,10,20 mg/kg) of ginsenosides Rg3.To observe the effect of ginsenoside Rg3 on tumor growth.Immunohisto-chemical-PAS staining was used to detect the effects of ginsenosides Rg 3 on vasculogenic mimicry of pancreatic cancer xenograft .and mRNA and protein expression of MMP 2、MMP9 were respectively evaluated by FQ -PCR and Western blot .Results The ginsenosides Rg3 can inhibit the growth of the tumor xenografts in nude mice .The inhibitory effect is the most obvious the 20 mg/kg of ginsenosides Rg3 group.The expression of MMP-2, MMP-9 were down-regulated compare with the control group , and the difference was signifi-cant;the Immunohistochemical -PAS staining showed the number of vasculogenic mimicry (+) and CD31 (+) were less than that in the control group .Conlusion Our results demonstrate that pancreatic vascular mimicry formation can be suppressed by Ginsenoside Rg 3 though reducing the expression of MMP -2, MMP-9 in our vivo experiments ,
