Deficiency of rpoS is the major factor leading to attenuation of Salmonella enterica serovar Choleraesuis vaccine strain C500

VernacularTitle:猪霍乱沙门菌疫苗株 C500毒力致弱的主要原因是 rpoS基因的缺失
Author: Lijuan XU ; Qiuchun LI ; Jie LIU ; Yachen HU ; Mingxin TAO ; Xiaolei XIE ; Shizhong GENG ; Xinan JIAO
Publication Type:Journal Article
Keywords: Salmonella Choleraesuis; vaccine; attenuation; Mirror Orientation Selection (MOS); Suppression Subtractive Hybridization (SSH)
From: Chinese Journal of Zoonoses 2015;(10):908-913
CountryChina
Language:Chinese
Abstract: Salmonella enterica serovar Choleraesuis strain C500 is a live attenuated vaccine that has been widely used in Chi‐na for over 50 years to prevent piglet paratyphoid .However ,as C500 is obtained by chemical methods ,the genetic background of this strain remained unclear .In this study ,we compared the genomic differences between the virulent reference strain C 78‐2 and C500 by suppression subtractive hybridization combined with the mirror orientation selection method (MOS‐SSH ) .Six genes (asr ,ydgF ,ydgD ,ydgE ,rpoS ,and ptsG) were lost in C500 strain .Using real‐time PCR analysis ,we demonstrated that the genes regulated by rpoS ,a vital transcriptional regulator playing an important role in Salmonella infection ,were downregulated in C500 .Additionally ,the virulence of the rpoS mutant strain C78‐2ΔrpoS was 100 000 times lower than the parental strain in BALB/c mice .So loss of rpoS gene is the major factor leading to the attenuation of C500 strain .