Molecular identification and resistant derterminants of Aeromonas sp .isolated from stool of human
10.3969/j.issn.1002-2694.2015.10.008
- VernacularTitle:气单胞属菌粪便分离株分子鉴定与耐药元件检测
- Author:
Xingbei WENG
;
Zuhuang MI
;
Tieli ZHOU
- Publication Type:Journal Article
- Keywords:
A eromonas;
molecular identification;
resistant determinant;
subtype;
mobile genetic element
- From:
Chinese Journal of Zoonoses
2015;(10):931-937
- CountryChina
- Language:Chinese
-
Abstract:
We investigated molecular identification of a group of 14 strains of Aeromonas sp .,and genetic background of re‐sistance to beta‐lactams ,aminoglycosides .From January to December 2012 ,14 strains of Aeromonas sp .were collected from stool from diarrheal patients in enteric clinics in Ningbo First Hospital in Zhejiang Province ,China .Then ,molecular identifica‐tion by 16SrDNA ,23 kinds of beta‐lactamase genes ,6 kinds of aminoglycoside modifying enzyme genes ,6 kinds of 16srRNA methylase genes ,and 6 kinds of mobile genetic elements were analyzed by PCR .In addition ,genotyping and sample cluster a‐nalysis were performed .Results showed that 10 strains of A .hydrophila ,1 strain of A .aquariorum ,A .sobria ,A .entero‐pelogenes ,A .punctata were confirmed by 16SrDNA sequencing and arithmetic .Five kinds of beta‐lactamase genes ,4 kinds of aminoglycoside modifying enzyme genes ,and 3 kinds of mobile genetic elements were positive .BlaAQU of strain No .4(AQU‐2) and strain No .11(AQU‐3) were new subtypes .It’s suggested that identification of Aeromonas sp .should be performed by molecular identification method .This group of 14 strains of Aeromonas sp .conferred multidrug resistance .
