Differential expression and function of chitinase 3-like-1 in macrophage stimulated by Sporothrix schenckii
10.3969/j.issn.1002-2694.2015.10.009
- VernacularTitle:类几丁质酶3蛋白1在申克孢子丝菌刺激巨噬细胞的表达和作用研究
- Author:
Lilin HUANG
;
Jin ZHANG
;
Wenchao GAO
;
Yuzhe LI
;
Liyan YUAN
;
Tailong HE
;
Huaiqiu HUANG
- Publication Type:Journal Article
- Keywords:
Sporothrix schenckii;
Candida albicans;
co-nidium;
chitinase 3-like-1
- From:
Chinese Journal of Zoonoses
2015;(10):938-942
- CountryChina
- Language:Chinese
-
Abstract:
We evaluated the differential expression and function of chitinase 3‐like‐1 in macrophage stimulated by Sporothrix schenckii and Candida albicans fungicidal ability of macrophage after stimulation with Sporothrix schenckii and Candida albi‐cans separately was detected .The expression of CHI3L1 gene in macrophage stimulated by Sporothrix Schenckii and Candida albicans was evaluated with real‐time PCR .The function of CHI3L1 protein in macrophages against the reproduction of Sporo‐thrix schenckii and Candida albicans was detected in vitro .Results showed that macrophages could engulf and kill Sporothrix Schenckii and Candida albicans in vitro .The expression of CHI3L1 gene in macrophage stimulated by Candida albicans was increased obviously .At the same time ,CHI3L1 protein can damper the reproduction of Candida albicans .However ,the ex‐pression of CHI3L1 gene was not elevated when macrophage was stimulated by Sporothrix schenckii and CHI3L1 protein played little role in reproduction of Sporothrix schenckii .The expression of CHI3L1 gene in macrophage was elevated after stimulation with Candida albicans ,but was not elevated with Sporothrix Schenckii .In correspondence with differential ex‐pression ,CHI3L1 in macrophages could impair the reproduction of Candida albicans but had a weak function on Sporothrix schenckii which might contribute to the pathogenesis of spo‐rotricosis .
