Role of N-acetylcysteine in protecting rats against contrast-induced acute kidney injury
10.3969/j.issn.1009-0126.2015.09.020
- VernacularTitle:N-乙酰半胱氨酸对大鼠造影剂诱导的急性肾损伤保护作用的研究
- Author:
Xiaoyu LI
;
Yongmin MAO
;
Naikuan FU
- Publication Type:Journal Article
- Keywords:
acetylcysteine;
contrast media;
C-reactive protein;
tumor necrosis factor-alpha;
kidney failure,acute;
malondialdehyde;
superoxide dismutase
- From:
Chinese Journal of Geriatric Heart Brain and Vessel Diseases
2015;(9):964-967
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of N‐acetylcysteine (NAC) in protecting rats against con‐trast‐induced acute kidney injury .Methods Forty‐five adult Wistar rats were randomly divided into control group ,model group and NAC group (15 in each group) .Their serum levels of creati‐nine ,CRP ,TNF‐a were measured before ,and 48 and 72 h after contrast medium injection .The an‐imals were killed 72 h after contrast medum injection .Their serum levels of malondialdehyde , SOD ,NO were measured .Eexpressions of Bax and Bcl‐2 were detected .Results The serum levels of creatinine ,CRP ,TNF‐a ,malondialdehyde and the expression level of Bax were significantly higher in model group and NAC group than in control group ,and in model group than in NAC group after contrast injection (P< 0 .05) .The serum levels of SOD and NO and the expression level of Bcl‐2 were significantly lower in model group and NAC group than in control group ,and in model group than in NAC group after constrast injection (P< 0 .05) .Immunohistochemistry demonstrated that the TGFβ1 expression level was significantly higher in model group and NAC group than in control group and was significantly lower in NAC group than in model group .Con‐clusion NAC can protect rats against kidney injury and reduce contrast‐induced acute kidney in‐jury by inhibiting inflammation ,oxidation ,apoptosis and cytokines .
