Application of Leucocyte Adherence Inhibition Test in Determining Immunological Activity of Ribonucleic Acid
- VernacularTitle:白细胞黏附抑制实验在核糖核酸药物免疫活力测定中的应用研究
- Author:
Wang CAN
;
Shao HONG
;
Chen GANG
- Publication Type:Journal Article
- Keywords:
Ribonucleic acidⅠ;
Ribonucleic acidⅡ;
Ribonucleic acidⅢ;
Leucocyte adherence inhibition test;
Immunological activity
- From:
China Pharmacist
2015;(11):1848-1850
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for determining the immunological activity of ribonucleic acid. Methods: Leucocyte adherence inhibition test ( LAI) was applied, and the important parameters of LAI including the mouse strain, drug concentration, treatment time, content of buffer solution and cell density were researched. The immunological activity of RNAⅠ, Ⅱand Ⅲ was re-spectively determined by the method. Results:Stable and reliable parameters were obtained: the sample concentration was 10 mg· ml-1 , the treatment time was 2 hours, Ca2+ and Mg2+ were necessary for the buffer solution, and the cell density was about 4 × 107 cell·ml-1 . The strain of mouse showed no effect on the results. As a result, the determination method for immunological activity was established. Using the method, the immunological activity of RNA Ⅰ,Ⅱand Ⅲ was determined 3 times, and the results met the re-quirements with RSD below 20%. Conclusion:The method is suitable for determining the immunological activity of RNA.
