The effect and mechanism of epithelial growth factor receptor (EGFR)in inducing vasculogenic mimicry formation

VernacularTitle:EGFR 对血管生成拟态的诱导作用及其相关机制
Author: Xi ZHANG ; Qimei ZHAO ; Chunyan WEI
Publication Type:Journal Article
Keywords: vasculogenic mimicry; hypoxia; glioma; EGFR/AKT/PI3K signaling pathway
From: Journal of Xi'an Jiaotong University(Medical Sciences) 2015;(6):739-742
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect and mechanism of EGFR in inducing vasculogenic mimicry (VM)formation.Methods Immunohistochemistry was used to detect the expression of EGFR protein and CD34-PAS double staining was used to detect the VM in gliomas paraffin blocks.The migration and VM formation of U87 cell in hypoxic and normoxic groups were detected by transwell and three-dimensional culture.Then we used Western blot to detect the expressions of EGFR,AKT and PI3K protein.Results The positive rate of EGFR expression in VM positive patients was 94.4%,which was significantly higher than that in VM negative patients (71.8%).In hypoxic group,the abilities of migration and VM formation were significantly higher those in normoxic group.The expressions of EGFR,AKT and PI3K were higher in hypoxic group.Conclusion VM formation can be induced by the activation of EGFR/AKT/PI3K signaling pathway.EGFR is very important for VM formation.