The effect of FASN on apoptosis in pancreatic cancer
10.7652/jdyxb201506011
- VernacularTitle:脂肪酸合成酶在胰腺癌细胞凋亡过程中的作用
- Author:
Changqing MIAO
;
Jing XU
;
Jun WANG
;
Huanchen SHA
;
Kaifei WANG
;
Xiaoqing LI
;
Bo PENG
- Publication Type:Journal Article
- Keywords:
fatty acid synthase (FASN);
pancreatic cancer;
growth;
apoptosis;
C75;
Caspase-3;
Bcl-2
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2015;(6):770-774
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of fatty acid synthase (FASN)on apoptosis in pancreatic cancer cell PANC-1 and the possible molecular mechanism.Methods Annexin V/FITC and flow cytometry were performed to detect the expression of FASN in pancreatic cancer PANC-1 after C75 treatment and the change of apoptosis in pancreatic cancer cell PANC-1 treated with C75.Quantity reverse transcriptase polymerase chain reaction (RT-PCR)and Western blot were used to measure the protein and RNA expressions of Caspase-3,bcl-2 and FASN.Results Inhibited by C75,the activity of FASN in pancreatic cancer cell PANC-1 was significantly decreased.Meanwhile,PANC-1 showed an increased apoptosis level in a dose-dependent manner (P < 0.05 ). Furthermore,after C75 inhibited FASN in pancreatic cancer cells,the protein and RNA expressions of Caspase-3 significantly increased (P <0.05)whereas the level of Bcl-2 reduced (P <0.05).Conclusion FASN is involved in the process of apoptosis in PANC-1 via Bcl-2 and Caspase-3.Therefore,FASN will provide a new target for the treatment of pancreatic cancer and generate better treatment efficacy.
