Effects of TGF-β1 on the migration,adhesion and proliferation of periodontal ligament stem cells
10.7652/jdyxb201506013
- VernacularTitle:转化生长因子β1对牙周膜干细胞迁移、粘附及增殖的影响
- Author:
Shuang WANG
;
Peixun FENG
;
Yue CHEN
;
Jianfeng SHI
;
Pei CAO
;
Haijuan ZHANG
- Publication Type:Journal Article
- Keywords:
periodontal ligament stem cell;
TGF-β1;
migration;
adhesion;
proliferation
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2015;(6):782-786
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of transforming growth factor β1 (TGF-β1 )on migration, adhesion and proliferation of periodontal ligament stem cells (PDLSCs)and explore the mechanisms of PDLSCs-induced periodontal remodeling.Methods PDLSCs were isolated and identified from human teeth.The effect of TGF-β1 on migration of PDLSCs was evaluated using transwell migration assay.Cells attachment assay was used to test the effect of TGF-β1 on the adhesion of PDLSCs.In addition,the effect of TGF-β1 on the proliferation of PDLSCs was evaluated by MTT and cell growth rate assay.Results The results showed that TGF-β1 induced the migration of PDLSCs in a dose-dependent manner,improved the adhesion and proliferation of PDLSCs.So we propose that TGF-β1 may promote periodium remodeling by inducing PDLSCs migration,following adhesion and proliferation in these areas.Conclusion This study demonstrated for the first time that TGF-β1 increases the adhesion and migration of PDLSCs in vitro .The signal pathway is involved in the TGF-β1-induced migration of PDLSCs and the mechanical-chemical interaction during the orthodontic periodontal remodeling will be researched in our further studies.
