Effect of calcium-sensing receptor on intracellular calcium, cell proliferation and migration of SGC-7901 cell line
10.13315/j.cnki.cjcep.2015.10.015
- VernacularTitle:钙敏感受体对SGC-7901细胞内钙、细胞增殖和迁移的影响
- Author:
Jian SUN
;
Xiaoqin LIU
;
Qi WU
;
Li LI
;
Hongtao ZHAO
;
Yankun HAO
;
Zhifang LANG
;
Hairong LUAN
- Publication Type:Journal Article
- Keywords:
stomach neoplasms;
calcium sensing receptor;
SGC-7901;
intracellular calcium;
cell proliferation;
cell migration
- From:
Chinese Journal of Clinical and Experimental Pathology
2015;(10):1140-1144
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To observe the functional expression of calcium sensing receptor ( CaSR) in human gastric cancer SGC-7901 cell line, the effect of CaSR on intracellular calcium, cell proliferation and migration of SCG-7901. Methods The expression and distribu-tion of CaSR were detected by Western blotting and immunofluorescence observation in SGC-7901. The intracellular concentration of free calcium ( [ Ca2+] i ) was determined by confocal laser scanning microscopy. MTT, flow cytometry and scratch test were used to an-alyze the impact of CaSR the proliferation and the migration capabilities of SGC-7901 cell. Results CaSR protein was expressed in SGC-7901. Extracellular calcium or calindol significantly increased the expression of [Ca2+]i, CaSR and E-cadherin;In addition, the migration capabilities were decreased. Conclusion CaSR is expressed in SGC-7901. The activation of CaSR induces the expression of E-cadherin, and decreases migration ability.
