The study of targeted blocking SDF-1/CXCR4 signaling pathway in vivo with T140 on SDF-1 and MMPs levels

VernacularTitle:T140体内靶向阻断SDF-1／CXCR4信号通路对SDF-1及MMPs的影响
Author: Kun WANG ; Fengkai ZHAO ; Yanlin LI ; Yang YU ; Huanyu GAO ; Yu XIAO ; Longteng LI ; Xiangjia YAN ; Di JIA
Publication Type:Journal Article
Keywords: T140; SDF-1/CRCR4 signaling pathway; Matrix Metalloproteinases; Cartilage degeneration
From: The Journal of Practical Medicine 2015;(19):3133-3136
CountryChina
Language:Chinese
Abstract: Objective To explore the effect of T140 on SDF-1 and MMPs levels through targeted blocking SDF-1/CXCR4 signaling pathway , and to investigate the function of T140 to prevent from cartilage degeneration. Methods Thirty-six 9-month-old male healthy Hartley guinea pigs were divided into three groups: experimental group(group A,n = 12),experimental control group(group B,n = 12) and blank control group (group C,n = 12). In the 2nd,4th,6th,8th,10th,12th week, the levels of SDF-1 in serum were quantified with ELISA. In the 12th week, mRNA levels of MMP-3,MMP-9 and MMP-13 in articular cartilages were measured with RT-PCR. Results The serum levels of SDF-1 of the group A decreased gradually but increased in group B and C. Group A had a statistical significance compared with group B and C at the same time point (P< 0.05).The mRNA levels of MMPs in group A were lower than group B and C (P < 0.05). Conclusion T140 could block the SDF-1/CRCR4 signaling pathway and decrease the secretion of SDF-1 and mRNA expression levels of MMPs and reduce the cartilage degeneration.