MiR-106b activate Wnt/β-catenin pathway in HCC cells
10.3969/j.issn.1006-5725.2015.19.007
- VernacularTitle:miR-106b在肝癌细胞中激活Wnt通路
- Author:
Hongyun JIA
;
Sicong HUANG
;
Haoyu CHEN
;
Yongjie SHI
;
Haiying HUANG
;
Xiaoyan DENG
;
Gang SHEN
- Publication Type:Journal Article
- Keywords:
HCC;
miR-106b;
Wnt/β-catenin
- From:
The Journal of Practical Medicine
2015;(19):3140-3142
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of miR-106b on Wnt/β-catenin pathway in HCC cells. Methods QGY-7703 and HepG2 cells were transfected with miRNA mimics or inhibitors. TOP/FOP luciferase ratio assay was used to test the Wnt/β-catenin pathway activity. The expression of downstream targeted genes of Wnt/β-catenin pathway were examined by Real-time PCR. The accumulation of β-catenin in nuclears were measured by Western blotting. Results Ectopic expression of miR-106b dramatically increased the average TOP/FOP ratio and the mRNA expression of downstream targeted genes in QGY-7703 and HepG2 cells. Compared with that in control cells , miR-106b over-expression promoted the nuclear β-catenin accumulation in QGY-7703 cells. Clonclusion MiR-106b activated Wnt/β-catenin pathway in HCC cells.
