Molecular epidemiology of Chlamydia trachomatis in outpatients visiting STD clinics in Fuzhou,China
10.3969/j.issn.1002-2694.2015.10.011
- VernacularTitle:福州市性病门诊人群生殖道沙眼衣原体的分子流行病学研究
- Author:
Jianxiong XIAO
;
Huirong WANG
- Publication Type:Journal Article
- Keywords:
Chlamydia trachomatis;
genotyping;
molecular epidemiology
- From:
Chinese Journal of Zoonoses
2015;(10):947-951
- CountryChina
- Language:Chinese
-
Abstract:
We investigated the distribution of Chlamydia trachomatis genotypes in the outpatients visiting STD clinics in Fuzhou City ,China .Between 2013 and 2014 ,a total of 2 019 urethral or endocervical specimens from susceptible genital C .trachomatis infections were collected and detected by using real‐time PCR assay kit .The C .trachomatis‐positive specimens were subsequently amplified with nested PCR for Omp1 region and analyzed by sequencing .Genotyping was performed by BLAST similarity search and phylogenetic tree analysis .Eighty‐three of the 86 C . trachomatis‐positive specimens were suc‐cessfully sequenced .Only one specimen was identified as multiple infection .Eight different genotypes were identified .The most prevalent was F (36 .59% ) ,followed by E (24 .39% ) ,D (12 .20% ) ,G (9 .76% ) ,J (9 .76% ) ,H (3 .66% ) ,K (2 .44% ) and B (1 .22% ) .Synonymous mutation rate and non‐synonymous mutation rate among Omp1 genes of different geno‐types were calculated .All genotypes but G showed dN/dS values of<1 .Genotypes F and E were commonest .Synonymous sub‐stitution was the major pattern of variation in the process of Omp 1 gene evolution .
