Effects of endoplasmic reticulum stress on hepatocellular injury in young rats fed with high-fat diet
10.7652/jdyxb201506002
- VernacularTitle:内质网应激在高脂饲料诱导幼年大鼠脂肪性肝损伤中的作用
- Author:
Liang LIU
;
Yanfeng XIAO
;
Chunyan YIN
;
Weiqin ZHANG
- Publication Type:Journal Article
- Keywords:
endoplasmic reticulum stress;
hepatocellular injury;
young rat;
high-fat diet;
nonalcoholic steatohepatitis(NASH)
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2015;(6):724-729
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of endoplasmic reticulum stress on liver damage in young rats fed with high-fat diet.Methods We divided 48 male weaned young rats randomly into high-fat diet group and control group,which were separately fed with high-fat diet and normal diet.After feeding 8,12 and 1 6 weeks,the body weight and visceral fat of the rats were measured.The serum liver function was measured.The morphology of livers was observed by HE and transmission electron microscopy. The mRNA expressions of ATF6 and GRP78 in hepatocytes were measured with RT-PCR.Results ① The body weight and visceral fat weight of rats in high-fat diet group increased compared with those in control group (P <0.05).② Alanine aminotransferase and aspartate aminotransferase in high-fat diet group increased slightly over time (P >0.05);alanine aminotransferase at week 1 6 was increased significantly compared with that in controls (P < 0.05 ).③ Liver cells in high-fat diet group had steatosis at week 8 and the steatosis became more serious between week 12 and week 1 6.④ In high-fat diet group at week 8 there were a large number of lipid droplets in the cytoplasm,and the cell structure was close to that of normal cells;rough endoplasmic reticulum was nearly normal and the ribosome was visible.At week 12 and week 1 6,besides a large number of lipid droplets,we could also see that some substances with line-like structure deposited in rough endoplasmic reticulum pool.⑤ The expressions of ATF6 and GRP78 mRNA in hepatocytes in high-fat group at weeks 8, 12 and 1 6 were significantly increased compared with those in control group (P <0.05). Conclusion High-fat diet in infants can cause visceral fat accumulation,fatty degeneration of hepatocytes and liver injury.ATF6-mediated endoplasmic reticulum may be closely related to the liver injury which results from highfat diet.
