Protective effect of granulocyte-colony-stimulating factor against chronic myocardial ischemia in rabbits
10.7652/jdyxb201506004
- VernacularTitle:粒细胞集落刺激因子对兔慢性心肌缺血的保护作用
- Author:
Qingbin ZHAO
;
Juan ZHOU
;
Yue WU
;
Yuling TIAN
;
Zhiquan LIU
- Publication Type:Journal Article
- Keywords:
granulocyte-colony-stimulating factor (G-CSF);
myocardial ischemia;
neovascularization;
CD34+
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2015;(6):735-738
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effects and mechanisms of granulocyte-colony-stimulating factor (G-CSF)on a rabbit model of chronic myocardial ischemia.Methods Myocardial ischemia models were created by partial ligation of the left anterior descending coronary artery in Japanese white male rabbits.Rabbits were subcutaneously injected with G-CSF (G-CSF group)or saline (control group)for 6 days after myocardial ischemia.The percentage of CD34-positive cells in the peripheral blood was evaluated by flow cytometry,and CD34-positive cells homing and vWF expression in the ischemic myocardium were determined by immunohistochemistry.Results Rabbits in G-CSF group had a higher survival rate than those in control group (P <0.05).Immunohistochemistry of the ischemic myocardium showed that compared with control group,G-CSF group had increased homing of CD34-positive cells on day 7 post-surgery,and more vessels on day 28 post-surgery by anti-von Willebrand factor staining.In addition,we observed an increase in the percentage of CD34-positive cells in the peripheral blood in G-CSF group.Conclusion G-CSF produces an obvious protective effect against chronic myocardial ischemia in rabbits by increasing stem cell mobilization,homing to ischemic myocardium and accelerating neovascularization.
