Protection of sodium tanshinone ⅡA sulfonate on adriamycin-induced myocardial cell injury
10.3969/j.issn.1006-5725.2015.19.008
- VernacularTitle:丹参酮ⅡA磺酸钠对阿霉素致心肌损伤的保护作用
- Author:
Wenjing ZHANG
;
Ping XIE
;
Peng SU
;
Yanling LI
- Publication Type:Journal Article
- Keywords:
Heart failure;
Sodium tanshinone ⅡA sulfonate;
Adriamycin
- From:
The Journal of Practical Medicine
2015;(19):3143-3146
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of sodium tanshinone ⅡA sulfonate(STS) on adriamycin-induced H9c2 cell apoptosis. Methods H9c2 cells were divided into four groups: the control group, the ADR group, the STS group, and the AS(ADR+STS) group. MTT assay were used to detect cell proliferation and cell death. The cell cycle of H9c2 cells were determined by flow cytometry assay. Protein expression of Bcl-2, Bax and Caspase-3 was detected by Western blot assay. Results Compared with the control group, cell proliferation was significantly reduced , and the number of the cells was significantly increased in the G0/G1 phase in the ADR group(P < 0.01, P < 0.05). At the same time, the expression of Caspase-3 was dramatically enhanced and the ratio of Bcl-2/Bax was significantly reduced (P < 0.05, P < 0.01) in the ADR group. However, pretreatment of STS increased H9c2 cell proliferation, decreased the number of the arresting cells, inhibited the expression of Caspase-3, and improved the ratio of Bcl-2/Bax (P < 0.05, respectively). Conclusions STS can attenuate ADR-induced myocardial cell apoptosis.
