Establishment of an orthotopic transplantation model of osteosarcoma labeled with three reporter genes
10.7644/j.issn.1674-9960.2015.10.006
- VernacularTitle:三报告基因标记骨肉瘤细胞及原位移植模型的建立
- Author:
Shanfeng ZHANG
;
Zhenxing LIU
;
Zhiwei YE
;
Zhonghua YANG
- Publication Type:Journal Article
- Keywords:
osteosarcoma;
U2-OS;
living imaging;
orthotopic transplantation;
models,animal
- From:
Military Medical Sciences
2015;(10):755-758
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an orthotopic osteosarcoma nude mice model that co-expresses green fluorescent protein( GFP) , red fluorescent protein ( RFP) and luciferase for the purpose of monitoring the growth of osteosarcoma and screening drug candidates against osteosarcoma .Methods Human osteosarcoma cells of U 2-OS were infected with lentivirus carrying reporter gene .The reporter gene expression was verified by fluorescent microscopy and bioluminescence imaging.The cells were transplanted into tibia of the nude mice and monitored by bioluminescence imaging .Results The reporter gene was stably expressed in U 2-OS cells.The growth and metastsis of osteosarcoma could be detected in nude mice.Conclusion The established orthotopic osteosarcoma nude mice model is an ideal model for investigating the mechanism of growth and metastasis of osteosarcoma and for screening drug candidates against osteosarcoma .
