Differential diagnosis of rat C6 glioma and inflammation with 18F-FDG, 11C-MET and 11C-CHO PET/CT imaging and their correlations with HIF-1α and VEGF
10.3760/cma.j.issn.2095-2848.2015.05.002
- VernacularTitle:18F-FDG、11C-MET和11C-CHO PET/CT对大鼠C6胶质瘤和炎性病变的鉴别诊断价值及与HIF-1α、VEGF的相关性研究
- Author:
Zhenguang WANG
;
Nan CHENG
;
Dacheng LI
;
Fengyu WU
;
Bin SHI
;
Chengcheng CHEN
;
Wei XUE
- Publication Type:Journal Article
- Keywords:
Glioma;
Inflammation;
Deoxyglucose;
Methionine;
Choline;
Tomography,emission-computed;
Tomography,X-ray computed;
Rats
- From:
Chinese Journal of Nuclear Medicine and Molecular Imaging
2015;35(5):340-345
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare 18F-FDG、11C-MET and 11C-CHO PET/CT in rat C6 glioma and inflammation models and observe their correlations with HIF-1α and VEGF expressions.Methods Thirtytwo male Wistar rats were included to bear both C6 glioma and turpentine oil-induced acute inflammation (AI).18F-FDG,11C-MET and 11C-CHO PET/CT were performed on rats.The SUVmax ratios of tumor-tomuscle (T/M),AI-to-muscle (AI/M) and tumor selectivity index (SI) were calculated.One-way analysis of variance and two-sample t test were used for statistical analyses.HIF-1α and VEGF expression was detected by immunohistochemical staining.Spearman correlation analysis was performed to evaluate the relationship between T/M ratios and the expressions of HIF-1α or VEGF.Results T/M ratios of 18F-FDG,11 C-MET and 11C-CHO in C6 glioma were 6.89±2.53,2.75±0.87,2.73±1.01,and the AI/M were 4.77±2.21,1.75±0.66,2.23±0.90 respectively.The 18F-FDG and 11C-MET uptake between C6 glioma and AI were significantly different(tFDG =2.133,tMET =3.267,both P＜0.05).The SIMET was significantly higher than SIFDG(t =2.600,P＜0.05).The 11C-CHO uptake between tumor and inflammation showed no significant difference(t=1.537,P＞0.05).T/M ratios of 18F-FDG and 11C-MET were positively related to HIF-1α and VEGF expressions(rs =0.725,0.637,0.621,0.764,all P＜0.05).The T/M ratio of 11C-CHO related only to VEGF (rs =0.682,P＜0.05).Conclusions 18F-FDG and 11 C-MET PET/CT may differentiate C6 glioma from AI,and 11 C-MET PET/CT seems more tumor-selective.11C-CHO PET is less valuable for the differential diagnosis.The 18F-FDG and 11 C-MET uptake of C6 glioma may be related to tumor hypoxia.All the three tracers could reflect tumor angiogenesis,but with different sensitiveness.
