Moderate hypothermia reduces hepatic ischemia-reperfusion injury
10.3760/cma.j.issn.1007-8118.2015.08.012
- VernacularTitle:亚低温对肝脏缺血再灌注损伤的防护作用研究
- Author:
Qifa YE
;
Long HU
;
Zhiping XIA
;
Wei WANG
;
Yanfeng WANG
;
Yan XIONG
;
Qiang TU
- Publication Type:Journal Article
- Keywords:
Moderate hypothermia;
Hepatic ischemia-reperfusion injury;
Cold-inducible RNA-binding protein
- From:
Chinese Journal of Hepatobiliary Surgery
2015;21(8):555-558
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of moderate hypothermia (MH) in liver ischemiareperfusion (IR) injury.Methods Male BALB/c mice (8 weeks old,n =15) were randomly divided into three groups:IR group:five mice subjected to 70％ hepatic IR (hepatic vascular triad above the bifurcation occlusion for 35 min before 24 h reperfusion) in normal temperature condition (37 ±0.5 ℃);MH + IR group:five mice were treated with MH (32 ±0.5 ℃) for 2 h before 70％ hepatic IR was performed;sham group:the other five mice were subjected to laparotomy and liver manipulations without vascular occlusion.AST and ALT in plasma were detected in all mice,and the morphological changes,cell apoptosis and the cold-inducible RNA-binding protein (CIRP) expression after MH in liver tissues were detected.Results Compared with IR group,the ALT and AST levels in MH + IR group were significantly decreased.In IR group,the liver morphology deteriorated with more severe hydropic degeneration and more cell apoptosis.In MH + IR group,the expression of CIRP began to increase after MH preconditioning.Conclusion MH preconditioning could protect against the liver ischemia-reperfusion injury.
