Cucurmosin combined with common chemotherapeutic drugs inhibited human pancreatic cancer cell line BxPC-3 in vitro
10.3760/cma.j.issn.1007-8118.2015.09.014
- VernacularTitle:南瓜蛋白联合常用化疗药物对人胰腺癌BxPC-3细胞的体外抑制作用
- Author:
Qiyu CHI
;
Heguang HUANG
;
Congfei WANG
;
Jieming XIE
;
Weize HU
;
Mingjuan FU
;
Minchao CHEN
- Publication Type:Journal Article
- Keywords:
Cucurmosin;
Pancreatic cancer;
Chemotherapeutic drugs;
Cell Proliferation;
Synergistic effect
- From:
Chinese Journal of Hepatobiliary Surgery
2015;21(9):629-632
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effect of cucurmosin (CUS) combined with the commonly used chemotherapeutic drugs for pancreatic cancer in clinical practice including Gemcitabine (GEM),Fluorouracil (5-FU),Paclitaxel (PTX) and Cisplatin (DDP) on cell proliferation of human pancreatic cancer cell line BxPC-3.Mehtods Sulforhodamine B (SRB) assay was used to detect the inhibition on the cell proliferation of BxPC-3 cells in vitro after the treatment of CUS combined with GEM,5-FU,PTX and DDP,respectively.Colony formation assay was also conducted and Jin' s formula was used to assess the synergistic effect of these combinations.Results The inhibition rate of CUS combined with GEM,5-FU,PTX and DDP were all higher than those of each drug alone (q ＞ 0.85),which became obvious in low concentrations.The colony formation inhibition rate of CUS combined with GEM,5-FU,PTX or DDP were all higher than each single drug treatment (q ＞ 1.15).Conclusion CUS could enhance the cell growth inhibition of GEM,5-FU,PTX and DDP in BxPC-3 cells in vitro with a good synergistic effect.
